|
KMID : 1094720090140010046
|
|
Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 1 p.46 ~ p.51
|
|
|
Enhanced Cutinase Production of Thermobifida fusca by a Two-stage Batch and Fed-batch Cultivation Strategy
|
|
Gangqiang He
Guanghua Huo
Liming Liu
Zhu Yang
Du Guo Cheng
Jian Chen
|
|
|
Abstract
|
|
|
|
In this study, cutinase production by Thermobifida fusca WSH03-11 was investigated with mixed short-chain organic acids as co-carbon sources to demonstrate the possibility of producing high value-added products from organic wastes. T. fusca WSH03-11 was cultured with different combinations of butyrate, acetate, and lactate with a purpose of increasing cutinase activity. The optimum proportion of butyrate, acetate, and lactate was 4:1:3. In batch cultivation, acetate and lactate were consumed quickly, while the consumption of butyrate was depressed in the presence of acetate with a concentration higher than 0.5 g/L. Based on these results, a two-stage batch and fed-batch cultivation strategy was proposed: a batch culture with acetate and lactate as the co-carbon sources in the first 10 h, and then a fed-batch culture with a constant butyrate feeding rate of 12 mL/h during 11~20 h. By this two-stage cultivation strategy, cutinase activity, dry cell weight, and consumption rate of butyrate were increased by 70%, 103.4%, and 4.3-fold, respectively, compared to those of the batch cultivation. These results provided a novel and efficient way to produce high value-added products from organic wastes.
|
|
|
KEYWORD
|
|
|
Thermobifida fusca, mixed acids, cutinase, cultivation strategy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|